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facs buffer  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc facs buffer
    Facs Buffer, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 2155 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/facs+buffer+dapi/DAPI/pm40316023-209-7-14
    Average 98 stars, based on 2155 article reviews
    facs buffer - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    FACS:

    Article Title: Unique pharmacology of mGlu homo- and heterodimers
    Article Snippet: .. Cells were washed twice with 1 mL of FACS buffer, then resuspended in 300 μL of FACS buffer containing 0.2 μg/mL DAPI (Cell Signaling Technologies). .. Samples were run on a FACSymphony A1 (BD Biosciences) following instrument compensation for Alexa Fluor 647 and PE/Cyanine7 collecting 20,000 events per condition.

    Article Title: The impact of subunit type, alternative splicing, and auxiliary proteins on AMPA receptor trafficking.
    Article Snippet: The cell pellets were resuspended in 100 μL of staining mix (10% FBS in FACS buffer) and 2 μL of allophycocyanin (APC) anti-HA.11 [clone 16B12] (BioLegend). .. Cells were resuspended in 100-200 μL of FACS buffer containing 0.2 ug/mL of DAPI (Cell Signaling Technologies) to assess viability. ..

    Article Title: Selective activation of FZD7 promotes mesendodermal differentiation of human pluripotent stem cells
    Article Snippet: .. Cells were resuspended in 500 mL of FACS buffer with 0.5 mg/mL DAPI (Cell Signaling Technology). .. The cell suspensions were analyzed using the FACS Fortessa (BD Biosciences) and the resulting FSC files were processed with the FlowJo software (BD Biosciences).



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    A Immunostaining of sagittal sections from a CS17 human embryo. Co-expression of NR2F2 (green) and SOX18 (blue) in the gut. scale bars 50 μm. B-B ”,and C-C ” Sections of proband liver (B) and duodenum (C) stained for CD31 (cyan), SOX18 (red arrows), NR2F2 (green arrows), and <t>DAPI</t> (purple). Orange and gold boxes denote zoomed in regions of individual vessels. Orange box represents vessel that shows cells co-expressing both NR2F2 and SOX18 in the same nucleus, whereas the gold box is a vessel with cells either expressing NR2F2 or SOX18. Scale bar = 50μm (B-C) and 10 μm (B’”-C””).
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    Image Search Results


    A Immunostaining of sagittal sections from a CS17 human embryo. Co-expression of NR2F2 (green) and SOX18 (blue) in the gut. scale bars 50 μm. B-B ”,and C-C ” Sections of proband liver (B) and duodenum (C) stained for CD31 (cyan), SOX18 (red arrows), NR2F2 (green arrows), and DAPI (purple). Orange and gold boxes denote zoomed in regions of individual vessels. Orange box represents vessel that shows cells co-expressing both NR2F2 and SOX18 in the same nucleus, whereas the gold box is a vessel with cells either expressing NR2F2 or SOX18. Scale bar = 50μm (B-C) and 10 μm (B’”-C””).

    Journal: bioRxiv

    Article Title: Inhibiting SOX18 with propranolol restores vascular integrity in NR2F2-driven malformations

    doi: 10.1101/2025.04.25.650344

    Figure Lengend Snippet: A Immunostaining of sagittal sections from a CS17 human embryo. Co-expression of NR2F2 (green) and SOX18 (blue) in the gut. scale bars 50 μm. B-B ”,and C-C ” Sections of proband liver (B) and duodenum (C) stained for CD31 (cyan), SOX18 (red arrows), NR2F2 (green arrows), and DAPI (purple). Orange and gold boxes denote zoomed in regions of individual vessels. Orange box represents vessel that shows cells co-expressing both NR2F2 and SOX18 in the same nucleus, whereas the gold box is a vessel with cells either expressing NR2F2 or SOX18. Scale bar = 50μm (B-C) and 10 μm (B’”-C””).

    Article Snippet: Cells were then washed with FACS buffer (1:5), and resuspended in 300μl FACS buffer + DAPI ((Miltenyi Biotec, # 130-111-570) for live/dead cell identification.

    Techniques: Immunostaining, Expressing, Staining